How are lysosomal processes involved in bone mineralisation?
The mechanisms that control the quantity of mineral crystals that are incorporated into the skeleton are poorly understood, even though this is a major contributor to bone strength. Our recent discoveries indicate that intracellular vesicles, including lysosomes, are involved in mineral secretion by osteoblasts (bone forming cells) and osteocytes (cells embedded in the bone). This project will use cell culture techniques, gene knockdown studies, and cell-based assays such as confocal immunofluorescence, molecular biology, reporter assays, and quantitative PCR techniques to determine the function of lysosomes in osteocytes and osteoblasts.
Prof Natalie Sims
Bone cell biology & disease
For further information about this project, contact: